人胃癌及其癌前病变的DNA、AgNORs定量分析

The Quantitative Pathological Studies on DNA and Argyrophilic Nucleolar Or-ganizer Region Associated Proteins of Human Gastric Carcinoma and Precan- cerous Lesions of Stomach

目的 对人胃癌及其癌前病变中DNA含量、AgNORs进行定量分析。方法 采用比色分析法测定胃癌20例,胃癌前病变12例及正常胃黏膜14例细胞核DNA含量;并通过银染技术对细胞核仁组成区AgNORs的数量、大小及形态进行观察。结果 正常胃黏膜、癌前病变、癌的DNA含量分别为(1.53±1.03)mg/L,(3.18±0.41)mg/L,(4.73±0.65)mg/L;AgNORs数量分别为1.57±0.24,3.24±1.08,4.43±1.60;平均最大直径分别为(1.01±0.17)μm,(1.41±0.38)μm,(2.52±0.62)μm;异形率分别为(10.8±1.2)％,(18.8±2.2)％,(36.4±3.4)%。各组间均有显著性差异。结论 胃癌及其癌前病变过程中DNA含量,AgNORs数量、大小、形态在胃癌发生发展过程中起重要作用。

Objective To investigate the quantitative pathological studies on DNA and argyrophilic nucleolar organizer region associated proteins of human gastric carcinoma and precancerous lesions of stomach. Methods The content of nuclear DNA was measured by colorimetric method in 14 nonnal stomach, 12 precancerous lesions of stomach and 20 gastric carcinoma;and using the argy-rophil technigue to observed the count , the range and the rate of heterodoxy of argyrophilic nucleolar organizer region associated proteins particles. Results The content of nuclear DNA was (1.53 ±1.03)mg/L,(3.18 ±0.41)mg/L,(4.73 ±0.65)mg/L; the count was 1.57±0.24,3.24 ±1.08,4.43±1.60; the range was (1.01 ±0.17)μm, (1.41 ±0. 38) μm, (2.52±0.62) μm and the rate of heterodoxy was (10.8 ±1.2)% ,(18.8 ±2.2)% ,(36.4 ±3.4)% in argyrophilic nucleolar organizer region of human normal stomach and precancerous lesions of stomach, gastric carcinoma. There were significanct difference between different groups. Conclusion The content of nuclear DNA; the count , the range and the rate of heterodoxy of argyrophilic nucleolar organizer region associated proteins particles play an important role in the course of occurring and development of gastric carcinoma.