2型糖尿病人血浆肿瘤坏死因子-α测定及意义

Determinations of plasma tumor necrosis factor-α level in type 2 diabetes mellitus and its significance

目的 探讨 2型糖尿病人血浆肿瘤坏死因子 - α(TNF- α)水平的变化。方法 选取正常对照组和 2型糖尿病 (DM2组 )为研究对象 ,再将 2型糖尿病组按体质量指数 (BMI) <2 5的分为 DM2 a组 ,≥ 2 5的分为 DM 2 b组 ;用酶联免疫法 (ELISA)测定各组的 TNF-α水平并比较。结果 正常对照组血浆 TNF-α为(0 .79± 0 .0 8)μg/L,DM 2 a组为 (1 .2 1± 0 .3 1 )μg/L ,比正常组升高 (P <0 .0 5 ) ;DM 2 b组为 (2 .46± 0 .5 7)μg/L,与正常组比较显著升高 (P <0 .0 1 ) ,DM2 a与 DM2 b相比 ,两者差异亦有显著意义 (P <0 .0 1 )。结论 2型糖尿病人血浆 TNF- α水平显著高于正常人 ,并与肥胖有一定的关系。

Objective To study the level Change of plasma tumor necrosis factor-α (TNF-α) in type type 2 diabetes.Methods The plasma Concentrations of TNF-α were determined by ELISA in 15 control subjects (Control group),and 24 type 2-diabetes(DM2 group),which were further subdivided into 2 subgroups based on BMI as DM2a group (BMI<25) and DM2b group (BMI≥25).Results The plasma TNF-α level was(0.79±0.08)μg/L in Control gronp;the TNF-α level in DM2a and DM2b all increased significantly as compared with Control group (P<0.05 and 0.01),and the TNF-α level in DM2b was significantly higher than that in DM2a (P<0.01).Conclusions The concentration of TNF-α increased significantly in type 2-diabetes as compared to normal subjects,and it was correlative with obesity.