重症肌无力患者胸腺细胞及外周血淋巴细胞Fas表达研究

Study on Fas Expression of Thymocyte and Peripheral Blood Lymphocyte of Patients with Myasthenia Gravis

目的 探讨重症肌无力(MG)的发病机制。方法 采用流式细胞术(FCM)分别检测MG患者胸腺细胞、外周血淋巴细胞(PBL)及地塞米松培养胸腺细胞的Fas表达。结果 MG患者PBL CD4、CD8细胞Fas表达率(分别为49％,34％;39％,34％)与对照组(分别为58％,25％;49％,24％)相比差异无显著性;MG患者胸腺细胞Fas表达率(11.20％,6.13％)较对照组(6.15％,1.84％)明显增高;地塞米松培养组Fas表达率MG组、正常人组分别为11.54％、8.62％和7.08％、3.13％,与空白对照组(MG组、正常人组分别为11.36％、6.87％和7.22％、2.86％)相比差异无显著性。结论 MG患者PBL,CD4、CD8细胞Fas表达率无明显增高,可能与自身反应淋巴细胞Fas和PBL FasL结合,发生程序化死亡有关。MG患者胸腺细胞Fas表达增高,可能参与MG发病机制。

Objective To investigate the pathogenesis of myasthenia gravis(MG). Methods Flow cytome-try(FCM) was adopted to examine the Fas expressions of thymocytes and peripheral blood lymphocytes (PBLs) of MG. Results There was no sigificant difference in the proportions of Fas+ PEL between MG patients(49% , 34%;39%,34% in CD4, CD8 respectively) and controls (58% ,25% ;49% , 24% in CD4, CD8 respectively). The proportion of Fas+ thymocytes of MG(11. 20% ,6. 13%) was significantly higher( P <0. 01) than controls (6. 15% .1. 84%). Both in MG and normal human Fas+ thymocytes, there was no significant difference( P > 0.05) between dexamethasone groups (11. 54% , 8. 62% ; 7. 08% , 3. 13% in MG, normal human respectively) and control groups(11. 36%,6. 87%;7. 22%,2. 86% in MG, normal human respectively). Conclusions The proportions of Fas+ in CD4, CD8 of MG were not significantly different from controls which might be associated with the correction of FasL of PBL. The proportion of Fas+ thymocytes of MG was significantly increased which might be involved in the pathogenesis of MG.