雌激素对帕金森病保护作用的实验研究

Experimental Study on the Protective Effect of Estrogen to Parkinson Disease

目的 探讨雌激素对帕金森病是否具有保护作用。方法 利用免疫组化染色(ABC)法计数酪氨酸羟化酶(TH)的阳性细胞数,用TUNEL法观察每视野下凋亡细胞的数目,比较各组的差异。结果 正常雌性小鼠造模(A)组比去势后造模(B)组中只给予生理盐水(B1)组、造模后去势(C)组中只给予生理盐水(C1)组的TH阳性细胞数目多,凋亡细胞数目少(P<0.05);B1组与C1组差异无显著性(P>0.05);B2组(B组中给予雌激素组)比B1组及C2组(C组中给予雌激素组)比C1组FH阳性细胞多,凋亡细胞数目少(P<0.05)。结论 雌激素对帕金森病具有保护作用和损伤修复作用,且与凋亡有关。

Objective To explore if estrogen could provide protective effect of Parkinson disease, and the relationship between estrogen protective effect and apoptosis of nigral neurons of mouse. Methods Using im-raunohistochemical method of avidinbiotin peroxidase complex (ABC), we observed distribution of positive cells of tyrosine hydroxylase (TH)-positive cells and fibres in the substantia nigra of the mouse. Using TUNEL we observed the number of apoptosis cell in every visual field, obtain the difference between the test group and controls. Results The number of positive cells of TH was larger and the number of apoptosis cells was smaller in the normal-female-mouse models group than in the group of making model after ovariosteresis and the group of making model before ovariosteresis( P <0. 05); respectively, in each group, the results was simillar between the group given estrogen and the controls. Conclusions Our study suggest that estrogen may have protective effect on the nigral neurons of Parkinson disease. What more, the protective effect might be associated with the apoptosis.