摘要
研究了AFLP标记在研究小香羊遗传多态性方面应用的可行性和该山羊个体基因组DNA的AFLP扩增结果。实验应用 1 0条AFLP引物 ,用PstⅠ酶切 ,对 1 5只小香羊基因组DNA进行AFLP反应 ,共获得1 1 3个AFLP标记 ,单引物获得的标记数在 2～ 1 9之间 ,小香羊群体相似系数AFLP研究结果为 0 91 3( 0 81 4～ 0 980 )。该研究为评价小香羊的遗传稳定性提供了相关的参数 ,准确评价尚待和其它品种对比研究后确定。
The AFLP markers were applied to detect genetic polymorphic loci in miniature Xiang goats.Ten AFLP primers were used, and genomic DNA from 15 goats were analyzed. A total of 113 AFLP markers were obtained, and the marker numbers of individual primer were between 2 to 19.The results indicate that the AFLP marker is suitable for analyzing genetic polymorphism in goat and that(2) the similarity index of population in miniature Xiang goats was 0 913(0 814 to 0 980).
出处
《动物学杂志》
CAS
CSCD
北大核心
2003年第5期45-49,共5页
Chinese Journal of Zoology
基金
国家自然科学基金资助项目 (No .30 0 70 12 0 )
关键词
小香羊
遗传多态性
遗传相似系数
AFLP
Miniature Xiang goat
Genetic polymorphism
Genetic similarity coefficient
AFLP (amplified fragment length polymorphism)
