含Calbindin-D28K mRNA的神经元在大鼠前脑中的分布—原位杂交组织化学法观察

LOCALIZATION OF THE NEURONS CONTAINING CALBINDIN-D28K mRNA IN THE RAT FOREBRAIN

本实验应用原位杂交组织化学技术,利用同位素标记的寡核苷酸探针,对大鼠前脑含Calbindin-D28 K mRNA的神经元的分布状况进行了详细的观察。结果发现在不同脑区或核团中,标记神经元的数量和标记强度各不相同。某些部位含许多强阳性神经元,如:前嗅核、大脑皮质、尾壳核、缰核、下丘脑、齿状回及中脑和杏仁复合体中的部分核团;然而,在另外一些脑区中,标记细胞呈中等阳性,如:嗅球的球旁细胞、盖带、梨状区内核、海马的CA1区中的锥体细胞层以及丘脑和杏仁核复合体中的部分核团。少数脑区中的标记细胞呈弱阳性,且数量较少,如;嗅结节、隔区、斜角带核等。这些结果表明含Calbindin-D28K mRNA的神经元在大鼠前脑中具有区域特异性分布特点,从而提示Calbindin-D28K在神经系统中的某些部位可能具有重要的作用。

Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D28K mRNA in the rat forebrain. Regions with strong labeling by the probe were as follows: the anterior olfactory nucleus, the cerebral cortex, the caudate-putamen, the accumbens, the habenular complex, the hypothalamus, the dentate gyrus and some nuclei of the thalamus and amygdala. However, some other regions such as the olfactory bulb, the tenia tecta,the medial geniculate nucleus, and some nuclei of the thalamus and amygdala contain the neurons labeled moderately. The neurons in the olfactory tubercle, the septum, the nucleus of the diagonal band and the lateral geniculate nucleus were weakly labeled. Therefore, the transcripts of calbindin-D28K gene show a region-specific localization in rat forebrain, indicating a important role of calbindin-D28K in these regions.