牛磺酸对镉染毒小鼠的保护作用

Protective Effects ofTaurine on Mice Exposed to Cadmium

[目的]初步探讨牛磺酸对镉所诱导的遗传毒性的拮抗作用。[方法]①小鼠骨髓嗜多染红细胞微核检测 :受试动物按单纯氯化镉剂量分为 :0.70mg、1.35mg、2.70mg三级及相应的加牛磺酸 (1000mg/kg)三组 ,另设阴性对照组和环磷酰胺组 ,共八组 ,分别计算其微核率。②小鼠精子畸形检测 :按单纯氯化镉剂量为 :0.50、1.00mg/kg二级及相应的加牛磺酸 (1000mg/kg)二组 ,另设阴性对照组和环磷酰胺组 ,共六组 ,计算其精子畸形率。③受试动物分单纯氯化镉组 (1.35mg/kg)、牛磺酸组 (1000mg/kg)及相应的牛磺酸加氯化镉组 ,再设阴性对照组 ,共四组 ,用原子吸收分光光度法测其肝脏、睾丸中的镉 (Cd)、铜 (Cu)、锌 (Zn)元素含量的变化。[结果]氯化镉各组脏器中Cd含量均高于阴性对照组 ;牛磺酸加氯化镉各组肝脏Cd、Cu、Zn含量均为明显高于氯化镉各组 ,睾丸中Cd含量(0.539μg/g)则明显低于氯化镉各组(0.683μg/g)。氯化镉各组小鼠骨髓嗜多染红细胞微核率分别为6.75‰、10.88‰、11.13‰和精子畸形率分别为40.88‰、59.00‰ ,明显高于阴性对照组 (微核率1.88‰、精子畸形率33.38‰ ) ,牛磺酸加氯化镉各组 ,微核率分别为4.75‰、5.13‰、5.63‰和精子畸形率分别为27.13‰、30.57‰ ,明显低于相应的氯化镉组。

To investigate the protective effects of Taurine(Tau)in mice acutely exposed to cadmium(Cd).①Aniˉmals were treated with CdCl 2 (1.35mg/kg),Tsu(1000mg/kg),Tau(1000mg/kg)and CdCl 2 +Tau respectively.The change of eleˉments(Cd,Zn,Cu)in liver and testicle was assayed by atomic absorption spectrophotometry.②There were CdCl 2 groups(0.70mg/kg,1.35mg/kg,2.70mg/kg)and corresponding CdCl 2 and Tau groups.Using micronuclei test of bone marrowin mice calculated the rate of polychromatic erythrocytes with micronuclei(MPCE)③Sperm shape abnormality test:Cd groups were given CdCl 2 (0.50mg/kg,1.00mg/kg).Cd+Tau groups were given corresponding Cd and Tau(1000mg/kg).Compared to the control group,Cd concentraˉtions increased significantlyin all cadmiumgroups.Cd,Cu and Zn concentrations increased significantlyin liverin CdCl 2 +Tau group comˉpared to Cd group;Cd concentrations decreased significantly in testicle in CdCl 2 +Tau group(0.539μg/g)compared to Cd group(0.683μg/g).CdCl 2 administered intraperitoneally at various doses could induce the marked increase in the rate of MPCE(6.75‰?10.88‰?11.13‰)and the rate of abnormalities of sperm(40.88‰?59.00‰)compared with control(1.88‰?33.37‰)in mice.But when tauˉrine was given simultaneously,the rate of MPCE(4.75‰?5.13‰?5.63‰)and abnormalities of sperm(27.13‰?30.57‰)significantly edcreased in each group,[Conclusion]Taurine could change the distribution of Cd,Cu and Zn in mice acutely epposed to cadmium and it had the protective effects on the genotoxicity of cadmium chloride.