丹皮多糖-2b对肾上腺素模型小鼠降血糖作用研究

Studies on hypoglycemic action of PSM-2b in adrenaline-induced model mice

目的 观察丹皮多糖-2b(PSM-2b)对肾上腺素模型小鼠血糖的影响。方法 采用300μg·kg-1肾上腺素腹腔注射复制小鼠高血糖模型。将小鼠随机分为三大组,各大组分别连续灌胃1周、2周或3周。每大组又分为六小组:对照组、模型组、阳性(降糖灵)组、PSM-2b大剂量(90mg·kg-1)组、中剂量(60mg·kg-1)组、小剂量(30mg·kg-1)组。按葡萄糖氧化酶法测血糖,按苯酚-硫酸法测肝糖元含量。结果 PSM-2b能够显著降低肾上腺素模型小鼠的高血糖,且其降血糖作用呈一定的量效关系。PSM-2b还能够促进血糖转化为糖元,显著提高模型小鼠肝糖元的含量。结论 PSM-2b可以通过抑制肾上腺素升血糖作用而降低血糖。

To observe the hypoglycemic effects of PSM-2b in adrenaline_induced model mice,mice model were induced by ip 300μg·kg-1 adrenaline(Adr).Themice were classified into three groups randomly.PSM-2b was administered to each mouse continuously for one,two or three weeks.Each group was classified into six subgroups respectively:control group?model group?positive and three experimental groups(PSM-2b 90mg·kg-1?60mg·kg-1?30mg·kg-1).Blood glucose and liver glycogen were measured by GOD method and phenol_sulphuric acid method. PSM-2b was able to decrease blood glucose level significantly in hyperglycemic mice by dosage_dependent manner.PSM-2b was also able to stimulate the synthesis of liver glycogen to increase the content of liver glycogen.PSM-2b was able to decrease the blood glucose level by inhibiting the effect of adrenaline.