MTHFR基因多态性与老年人脑梗死的相关性研究

The association of polymorphism in Methylenetetrahydrofolatereductase gene with senile cerebral infarction

目的 探讨N5,N10 亚甲基四氢叶酸还原酶 (MTHFR)基因多态性与老年人脑梗死的相关性及预后分析。 方法 应用多聚酶链反应 限制性内切酶片段长度多态性技术 (PCR RFLP)对 90例老年性脑梗死 (脑梗死组 )和 10 1例正常老年人 (对照组 )进行MTHFR基因多态性分析并进行基因型及等位基因频率计数。 结果 脑梗死组TT型基因频率及T等位基因频率分别为 2 6 7%和 49 4% ,对照组分别为 12 8%和 44 0 % ,经 χ2 检验 ,2组间有统计学意义 (P <0 0 1)。

Objective To observe the association of polymorphism in methylenetetrahydrofolate reductase(MTHFR) gene with senile cerebral infarction. Methods MTHFR gene was detected by PCR-RFLP in 90 cases of senile cerebral infarction (infarction group) and 101 cases of healthy elderly subjects(control group).The frequency of MTHFR genotype and alleles was counted and the polymorphism was analyzed. Results The frequency of TT genotype and T alleles was 26.7% and 49.4% in infarction group,12.8% and 44.0% in control group.Significant association was observed inter-group(P<0.01). Conclusions There was an association between polymorphism in MTHFR gene and senile cerebral infarction.