有机膦除草剂毒性对作物蛋白质组分变化的研究

Toxicity of Organo-Phosphine Herbi cide on Changes of Protein Compositions in Crops

利用有机膦除草剂甲基胺草膦(Amiprophos-methyl,APM)和草甘膦(Glyphosate,GPS),研究APM和GPS对黑麦蛋白质组分变化的影响。实验结果表明,在APM浓度为1.2mg·L-1,处理时间为24h时,GPS为1000mg·L-1,处理时间为26h时,通过双向SDS-PAGE检测,在黑麦根系中有多种蛋白质组分的增加、减少,或者其含量发生变化,在大麦幼苗中诱导出一个28KD/pI6.4的特异蛋白质组分,而在黑麦根系中有一个31KD/pI6.6的特异蛋白质组分丢失,这些蛋白质组分的变化可能与除草剂的毒害作用有关。1.2mg·L-1的APM引起黑麦、大麦蛋白质组分产生变化的临界时间为24h,而草甘膦对黑麦、大麦蛋白质组分产生变化的临界浓度和时间分别是1000mg·L-1和26h。

The effects of the organophosphine herbicide amiphrophos -methyl(APM)and glyphosate(GPS)were studies by eval-uation of change of protein of crops(Secale cereale and Hordeum vulgare).The results were as follows:in term s of the changes of proteins by 2D /SDS -PAGE,when the tr eatment concentration and time of APMand GPS were 1.2mg ·L -1 ,24h and 1000mg ·L -1 ,26h,we found that some proteins appeared,some proteins disappeared,o thers contents of proteins changed.Especially,one protein(28KD /PI6.4)was induced in seedlings of Hordeum v ulgare,while the other protein(31KD /PI6.6)was disappeared in roots of Secale cereale,suggesting that it was likely connected with herbicide treatment.Above all,protein compositions were changed when root tips and seedlings were treated with 1.2mg ·L -1 APMfor 24h.in Secale cereale and Hordeumvulgare;while the treatment concentration and time (1000mg ·L -1 and 26h)of GPS were consequently thought to t he threshold concentration and time for bringing about changes of compositions.