建立评价当归质量的HPLC指纹图谱分析方法

Establishment of HPLC-fingerprint analysis for the quality assessment of Angelica sinensis

目的 建立评价当归质量优劣的指纹图谱分析方法。方法 采用高效液相色谱分析 ,HypersilC18柱 (2 0 0mm× 4 .6mm ,5 μm) ,分别以乙腈 0 .0 1%磷酸水溶液 (10∶90 )、甲醇 乙腈 水 (10∶30∶6 0 )为流动相 ,流速为 0 .8mL·min-1,检测波长 32 0nm ,测定了 36批不同产地的当归药材的HPLC指纹图谱。结果 根据聚类分析和相似度分析处理结果 ,将当归药材分为两类 :合格药材与不合格药材。结论 本方法简便、快捷、可靠 ,可用于当归的质量评价。

OBJECITVE: To establish the HPLC-fingerprint analysis for the quality control of Angelica sinensis. METHODS: The HPLC method was used with Hypersil C18 column (200 mm × 4.6 mm, 5 μm). The mobile phases were consisted of acetonitrile-0.01% phosphoric acid (10:90) and methanol-acetonitrile-water (10:30:60) respectively. The flow rate was 0.8 mL&middotmin-1. The UV detection was performed at 320 nm. 36 batches of Angelica sinensis were determined. RESULTS: The 36 batches of Angelica sinensis were classified to be qualified and unqualified based on the results of cluster analysis and similarity analysis. CONCLUSION: The method was simple and reliable, and it was capable of effectively controlling the quality of Angelica sinensis.