江苏省艾滋病病毒感染的分子流行病学研究

A molecular epidemiological study on the human immunodeficiency virus infection in Jiangsu province

目的 了解江苏省不同人群中艾滋病病毒 (HIV )各亚型毒株的流行情况和传播规律。方法 收集HIV感染者及患者的流行病学资料 ;无菌采集HIV感染者或艾滋病 (AIDS)患者抗凝全血标本 5ml,提取前病毒DNA ,用巢式聚合酶链反应 (nested PCR)扩增膜蛋白基因的C2～V3区 ,进行序列测定 ,鉴定病毒亚型。用威斯康星GCG软件进行共享序列、基因离散率的计算和毒株的聚类分析。结果 截止 2 0 0 1年底 ,江苏省已发现HIV 1中的A、B、C、D、E五种亚型和一个B亚型变种(B’)流行 ,C(占 40 .48% )和B’(3 8.10 % )亚型为主要流行株 ;静脉吸毒感染人群中 86.67%为C亚型 ,采供血和受血感染人群中 91.67%为B’亚型 ,在性途径传播人群中所有六种亚型均有 ,且分布较为均一。结论 有偿献血人群中B’亚型毒株由邻省传入 ,吸毒人群中C亚型毒株的传入主要与新疆籍流动人口的介入有关 ;多种亚型的并存说明江苏省存在着适宜AIDS流行的各种危险因素 ;提示今后在药物治疗。

ObjectiveTo identify subtypes of human i mm unodeficiency virus 1 (HIV-1) strains and their distribution, infection source s, and the trends of HIV infection in Jiangsu province. Methods Anticongulated bloods from 46 infected persons we re collected to separate previrus DNA. HIV-1 env genes were then amplified by nested-PCR and sequenced for their C2-V3 region so as to identify subtypes. The analysis of consensus sequence, genetic distance and phylogenetic tree were conducted with GCG software. ResultsBy the end of 2001, there had been six subtypes o f HIV-1 strains identified in Jiangsu province: A,B,B’, C, D and E. The pred ominant subtypes were C(accounting for 40.48 %) and B’(accounting for 38.1 0 %). Subtype C accounted for 86.67 % among injecting DUs while subtype B’ accounted for 91.67 % among commercial blood donors and receivers. ConclusionSubtype B’among commercial blood donors was b rought to Jiangsu from neighboring provinces. The outbreak of HIV-1 infection among local DUs was caused by subtype C from Xinjiang province. Findings from HIV/AIDS molecular epidemiologic study suggest that it is challenging for Jia ngsu to treat patients, apply vaccine, prevent and control AIDS in the future.