一起引发手足口病流行的肠道病毒71型的分子特征

Nucleotide Sequence of Enterovirus Type71 Isolated from Hand-foot-and-mouth Disease Patients

上海市某幼儿园 2 0 0 0年 10～ 11月发生手足口病 (HFMD)流行 ,从患儿粪便标本中分离到 13株肠道病毒 71型 (EV71) ,血清学实验证明 ,所分离的病毒为此次HFMD流行的病因。对其中的 4株病毒 (SHANGHAI EV71 H2 5、SHANGHAI EV71 H2 6、SHANGHAI EV71 F1和SHANGHAI EV71 F2 )进行了VP1区全基因核苷酸序列测定 ,并做了比较分析。 4株EV71VP1区全基因均为 891个核苷酸 ,编码 2 97个氨基酸 ,与检索到的广东省深圳市和台湾省HFMD病人EV71分离株同源性较高 (>94 % ) ,与脑炎EV71分离株 (BrCr株 )同源性低 (<83% )。种系发生树分析亦表明 ,上海市的 4株EV71与深圳株 (AF30 2 996 EV71 SHZH98)和台湾株 (AF2 86 5 31 FV71 TW 98)亲缘关系较近 ,与BrCr株亲缘关系较远。因此上海市HFMD 4株EV71分离株为本地流行株。

In the year of 2000,there was an endemic of hand-foot-and-mouth disease(HFMD)in a kindergarten in Shanghai From the stool specimens,13 out of 25 specimens were positive of enterovirus type71(EV 71 ) The serological test demonstrated the isolated virus was the pathogen of the HFMD We determined the VP1 nucleotide sequences of strains (H25,H26,F1,F2)isolated from the HFMD pateints,and compared them with the sequences of related enterovirus type71 The complete nucleotide sequences of region VP1 of the 4 strains were all 891 nucleotides long coding 297 amino acids In comparison with the VP1 sequence of two EV 71 strains isolated separately from hand-foot-and-mouth disease patients in Shenzhen and Taiwan in GenBank through internet, the homogeneity of nucleotide sequence was more than 94%,and was low of 83% as compared with BrCr strain The 4 EV 71 strains isolated from hand-foot-and-mouth disease patients in Shanghai were endemic strains