非小细胞肺癌诱导型一氧化氮合酶表达及其与肿瘤细胞增殖关系的研究

Relationship between expression of inducible nitric oxide synthase and tumor cell proliferation in non-small cell lung cancer

目的:探讨非小细胞肺癌(NSCLC)癌组织诱导型一氧化氮合酶(iNOS)的表达及其与肿瘤细胞增殖的关系。方法:采用免疫组织化学SP法对64例非小细胞肺癌术后石蜡固定标本及12例正常肺组织标本中iNOS及Ki-67抗原的表达情况进行检测,观察iNOS的表达结果与NSCLC临床病理特征及细胞增殖活性之间的关系。结果:NSCLC中的iNOS表达阳性率为81.25%,正常肺组织中无iNOS表达,iNOS的表达程度与NSCLC的病理类型、分化程度、PTNM分期、肿瘤直径、吸烟与否无关,与淋巴结转移情况呈正相关;Ki-67抗原在正常肺组织中呈低表达,在NSCLC中呈高表达,其表达强度与NSCLC的病理类型、吸烟与否无关,与分化程度、PTNM分期、肿瘤直径、淋巴结转移情况呈正相关;NSCLC癌组织iNOS表达水平与Ki-67抗原表达强度呈正相关关系。结论:NSCLC中iNOS高表达与其生长、淋巴结转移有密切关系,检测其表达情况可预示病情进展;Ki-67抗原的表达强度能够很好的反映癌细胞的增殖活性,可作为判断恶性程度、转移的客观指标;癌细胞中iNOS诱导产生的NO对肺癌细胞的增殖有促进作用。

Objective:To investigate the expression of inducible nitric oxide synthase(iNOS)and Ki-67antigen in non-small cell lung cancer (NSCLC),and to study the irrelationship in the occurrence and the prognosis of NSCLC.Method:12normal samples of lung tissue and64paraffin-embedded samples from surgically treated patients with NSCLC were investigated by immunohistochemistry (S-P),and Spearman rank correlation was used to analyze the irrelationship.Result:In the case of NSCLC i-NOS expression was very high,with a positive rate of81.25%.No expression of iNOS in the normal lung tissues.The iNOS expression had no correlation with the degree of differentiation,PTNM stage,pathologic classification,and smoking habit,but had correlation with lymph node metastasis (P<0.01).The expression of Ki-67was low in the normal lung tissues,but very high in the cancer cells of NSCLC,bearing correlation the degree of differentiation,PTNM stage,lymph node metastasis,and sizes of tumors(P<0.01),but not with pathologic classification,and smoking habit.There was also a positive correlation between iNOS expression and Ki-67antigen expression in NSCLC(P<0.01).Conclusion:Since the expresses of iNOS in NSCLC correlates to the growth of tumor,and lymph node metastasis,detection of iNOS expression is of value in the assessment the NSCLC progression.The expression of Ki-67which may well reflect the proliferation of cancer cells can be used to assess the degree of ma-lignancy and metastasis.The large quantity of NO induced by iNOS can accelerate the proliferation of cancer cells.