丹参注射液及其活性成分与低强度He-Ne激光联用对牛中性粒细胞呼吸暴发的抑制效应

Effects of Salvia Miltiorrhiza Injection Combined with Low Intensity He-Ne Laser Irradiation on the Respiratory Burst of Bovine Neutrophils

目的 :丹参注射液与低强度He Ne激光联用在临床上治疗缺血、缺氧性心、脑血管疾病取得良好疗效 ,但其机制尚未完全阐明。观察丹参注射液及其活性成分与低强度He Ne激光联合作用在细胞水平的效应 ,以进一步探讨其抗缺血、缺氧性损伤的机制。方法 :应用化学发光法检测了丹参注射液及其主要活性成分丹参素和丹参酮分别与低强度He Ne激光联用对牛中性粒细胞(PMN)呼吸暴发的影响。结果 :低强度He Ne激光、丹参注射液、丹参素、丹参酮都能对PMN的呼吸暴发产生抑制效应 ,丹参注射液或其活性成分与低强度He Ne激光联用抑制效果更明显。结论 :丹参注射液或其活性成分与低强度He Ne激光联用能够协同抑制PMN的呼吸暴发。

Objective:Low intensity He Ne laser irradiation combined with Salvia miltiorrhiza injection(SM)can treat ischemia and anoxemia of heart and brain well in clinic,but the mechanism remains obscure.Primary study on cellular level is needed.We evaluated the effect of low intensity He Ne laser irradiation combined with SM,and two of its effective components,Danshensu and Tanshinone on the respiratory burst of bovine neutrophils,respectively.Methods:Neutrophils are divided into 4 groups:(1)control;(2) He Ne laser irradiation at a dose of 1000J/m 2;(3) cultured in SM or Danshensu or Tanshinone (8μl) for 10 minutes;(4) cultured in SM or Danshensu or Tanshinone (8μl) for 10 minutes then He Ne laser irradiation at a dose of 1000J/m 2.Results:He Ne laser,SM,Danshensu,Tanshinone,or the combination of He Ne laster and SM or Danshensu or Tanshinone,all can inhibit the respiratory burst of neutrophils evidently.The effect of the combination of laser and Chinese traditional medicine is more marked than that of only laser or SM or Danshensu or Tanshinone.Conclusions:These results suggest that He Ne laster and SM can inhibit the respiratory burst of neutrophils effectively and they have cooperative effect.