摘要
本文用反相高效液相色谱法测定了利福定在人体尿液中的浓度。色谱条件为:Shimpack CLC-TMS 5μm柱(150×4.6mm);0.01M磷酸二氢钾缓冲液—乙腈(50:50)为流动相;检测波长316nm.尿中利福定在0.2～20μg/ml的范围内呈线性关系。有机溶媒提取率为93%.用本法测定了利福定固体分散物相对于原药的生物利用度为147.7%.
A Rp-HPLC method has been developed for the quantitative determination of rifandin in urine. The chromatographic conditions were: a Shimpack CLC-TMS column (150×4.6mm),0.01M KH_2PO_4-Acetonitrile(50:50) as the mobile phase, 316nm of wave lengh. Good linear range was 0.5～20μg/ml. Extration ratio was 93%. The Bioavailability of rifandin dispersion against the original drug was 147.7%.
