绿清灵致突变性和亚慢性经口毒性的研究

Experimental research on mutagenicities and subchronic oral toxicities of Luqingling

目的 研究农药绿清灵的致突变性和亚慢性经口毒性。方法 按GB15670 -1995的方法进行Ames试验、小鼠骨髓微核试验、小鼠睾丸精母细胞染色体畸变试验和大鼠亚慢性经口毒性试验。结果 Ames试验各条件下均为阴性。各剂量组骨髓微核细胞率和睾丸精母细胞染色体畸变率与阴性对照组比较差异均无显著性 (P >0 0 5)。亚慢性经口毒性试验中 ,各剂量组在一般状态、体重、血常规、血液生化、脏器系数等指标均基本正常 ;高剂量组动物部分脏器 (心、肝、肾、肺、脾、脑 )和中剂量组动物部分动物脏器 (心、肝、肾、肺 )有不同程度的病理表现 ;经综合分析 ,绿清灵在本试验对SD大鼠亚慢性经口的最大无作用剂量为 4 2mg·kg- 1·d- 1。结论 本受试物在本试验剂量下无致突变作用 。

Objective To study the mutagenicities and subchronic oral toxicities of the pesticide Luqingling. Methods Based on the National Standard GB15670-1995, Ames test, bone marrow micronucleus test in mice, chromosome aberration test in mice testicle spermatocytes and subchronic oral toxicity test in rats were conducted.Results A negative result was observed in Ames test at all dose groups, compared with the controlled. No significant differences ( P >0 05) were observed in micronuclei rates and chromosome aberration rates between the controlled and all dose groups in bone marrow micronucleus test and chromosome aberration test. In the course of the subchronic test, no obvious toxic response was observed in the treated animals. Other indexes including body weight, blood routine examination, blood biochemical examination relatively remained normal. Histopathological examinations revealed that various changes were found in the high dose group (heart, liver, spleen, lung, kidney and brain) and in the medium dose group (heart, liver, kidney and lung). There were no obvious pathological changes in the low dose treated animals. The 90 day oral no observed adverse effect level (NOAEL) of Luqingling in SD rats was 4 2 mg·kg -1 ·d -1 . Conclusion Luqingling is regarded as a non mutagenic pesticide under the tested doses. The NOAEL of Luqingling can be used to evaluate the safety of the pesticide and to provide basis for further studies.