肿瘤相关粘蛋白MUC1及其mRNA与卵巢上皮性肿瘤关系的研究

Alterations of tumor-associated mucin MUC1、MUC1mRNA in human ovarian epithelial neoplasms

目的 :初步探讨肿瘤相关粘蛋白MUC1基因及其蛋白与卵巢上皮性肿瘤的关系。方法 :采用原位杂交法和免疫组化ABC法 ,检测 6 5例卵巢上皮性肿瘤组织和 8例正常卵巢组织MUC1mRNA及其蛋白的表达。结果 :MUC1mRNA和MUC1阳性表达皆位于胞浆和胞膜 ,多呈灶状分布。MUC1mRNA和MUC1在卵巢癌中的检出率分别为 75 .6 % ,80 .0 % ,显著高于良性上皮性肿瘤的 4 0 % ,4 5 %和正常卵巢组织的 2 5 .0 % ,37.5 % (P <0 .0 0 5 )。二者高表达与卵巢癌的期别晚、组织分化差及淋巴结转移有关。结论 :MUC1作为新型肿瘤标志物 ,与卵巢癌的发生。

Objective:To explore the expression of tumor associated mucin MUC1,MUC1 mRNA in human ovarian epithelial neoplasms.Methods:Immunohistochemistry and in situ hybridization were used to determine the expressions of MUC1,MUC1mRNA in human ovarian epithelial carcinogenesis.Results:MUC1mRNA and MUC1 protein positive dark blue and brown yellow granules were mainly intracytoplasmic.The positive cells were chiefly distributed in focuses.The positive expression rate of MUC1mRNA and MUC1 protein in ovarian epithelial carcinoma were 75.6%,80%,significantly higher than that in benign (40%,45%) and normal ovarian tissues(25%,37.5%)(P<0.005).The two high expressions were significantly associated with higher vicious degree of ovarian carcinoma,poor differentiation,and lymph nodes metastasis.Conclusion:As a new tumor mark,the high expression of MUC1 is well related with the generation and development of ovarian carcinoma.