活血化瘀化痰利湿法对肥胖性脂肪肝大鼠胰岛素敏感指数和肿瘤坏死因子的影响

Effect of Huoxue Huayu and Huatan Lishi on Insulin Sensitivity Index and TNF of Fattv Liver Rats

目的:探讨活血化瘀、化痰利湿法改善肥胖性脂肪肝大鼠胰岛素抵抗(IR)状态的机制,为临床中医辨证治疗脂肪肝提供实验依据。方法:32只健康雄性SD大鼠随机分为正常组、造模组、西药组、中药组。除正常组饲以普通饲料外,其余各组均饲以高脂饲料。造模4周后,上述4组分别以生理盐水、生理盐水、0.08％东宝肝泰混悬液、中药煎剂灌胃。实验第16周测定血清和脂肪组织中肿瘤坏死因子-α(TNF-α)及血糖、胰岛素等。并取肝组织标本病理切片。结果:①胰岛素敏感指数(ISI):治疗组较造模组显著提高(P<0.05);中药组与正常组相比无显著性差异(P>0.05),与西药组相比有明显差异性(P<0.05)。②血清和脂肪组织TNF-α:治疗组与造模组相比有显著性差异(P<0.01),治疗组与正常组相比无明显差异性(P>0.05),中药组与西药组相比无明显差异(P>0.05)。③肝脏病理切片:正常组大鼠肝脏无异常病变、模型组大鼠肝脏均出现不同程度的肝脂变。各治疗组大鼠肝脏脂变程度明显减轻。结论:活血化瘀、化痰利湿法能够降低脂肪细胞TNF-α的表达,提高肥胖性脂肪肝大鼠ISI,改善IR状态;改善肝细胞的病理损伤状况,从而达到抗脂肪肝的目的。

Objective: To observe the influence of the therapy promoting blood circulation to remove blood stasis, removing phlegm to decrease wet on obesity correlated fatty liver on weight, insulin sensitivity index and Tumor Necrosis Factor-a concentration in serum and TNF-α in fatty tissue. Methods: 32 SD male rats in health were divided into 4 groups at random: Normal control group, Model control group, Deng Bao Gan Tai group (DBGT group), and Herbal therapy group. Except normal control group, the other three groups were fedwith high caloricity and high fat diet. After 4 weeks later, the rats were treated respectively with sodium chloride, sodium chloride, 0.08percent of DBGT liquid and Chinese medicine. At the end of the 16 weeks, on foods were fed with the rats in 24 hours. Killed rats and fetched blood and then the FBS, INS, TNF-α were measured. The structural change of liver tissue was also obseruve by using the light microscopy. Conclusion: The results were analyzed statistically in F and Q test. Results: (1)Insulin sensitivity index: The treatment group was obvious raised comparing the model control group ( P < 0.05 ). There was obvious difference between the herbal control group and the DBGT group, but on difference with the normal control group (P>0.05). (2)The TNF-α: The treatment group was different with the model control group (P<0.01) but no difference with the normal control group (P>0.05). There was no difference between herbal control group and the DDBGT group. (P>0.05). The TNF-α in fatty tissue is the same as it in serum. (3)Fatty liver pathological change slice: There is no pathological change in the normal control group. But there is grease pathological change in the liver of model control group. The degree of the grease pathological change in the treatment groups is obvious relived. Conclusion: The therapy of promoting blood circulation to remove blood stasis, removing phlegm to decrease wet can lowered blood sugar, insulin, and decrease the TNF-α in fat tissue and raised the ISI of obesity correlated fatty liver. The therapy can improve the level of liver cell pathological change and insulin resistance.