Fas,FasL在喉癌中的表达及其临床意义

The expression of Fas,FasL in laryngeal carcinoma and its clinical significance

目的 :研究Fas ,FasL在正常喉黏膜及喉癌组织中的表达及意义。方法 :采用SABC免疫组化法检测 5例正常喉黏膜及 4 0例喉癌组织的Fas ,FasL表达。结果 :Fas在 5例正常喉黏膜中全部表达 ,在 4 0例喉癌中阳性表达 16例 ,阳性率为 4 0 % ,高分化组、临床Ⅰ、Ⅱ期组和未复发组Fas表达阳性率显著大于低分化组 ,临床Ⅲ、Ⅳ期组和复发组 (P<0 .0 5 ) ;FasL在 5例喉正常黏膜中均未见表达 ,在 4 0例喉癌组织中均见不同程度的表达 ,喉癌组织FasL表达强度与喉癌组织学分级、临床分期呈正相关 (r′s =0 .8392 ,0 .86 85 )。结论 :在喉癌的发生与演进过程中存在Fas丢失和FasL表达上调 ,检测Fas ,FasL表达对评价喉癌的恶性程度 。

Objective: To study the expression of Fas,FasL in laryngeal carcinoma and the role of them in the pathogenesis of laryngeal carcinoma . Method: With immunohistochemical staining techniques, 5 cases of normal laryngeal mucosa and 40 cases of laryngeal carcinoma specimen were detected for the expression of Fas and FasL. Results: 5 cases of normal laryngeal mucosa were all Fas-positive. The Fas-positive rate in the laryngeal carcinoma cases was 40%(16/40). The Fas-positive rate of the high differentiation,clinical Ⅰ,Ⅱ stage and unrecurrence group was higher than that of the low differentiation,clinical Ⅲ,Ⅳ stage and recurrence group(P<0.05), and was not relative to the site of the tumors(P>0.1).The normal laryngeal mucosa was FasL-negative, but the laryngeal carcinoma specimen was FasL-positive. There was positive correlation between the degree of the FasL expression and the histological grade,clinical stage (r′s=0.8392;r′s=0.8665).Conclusion: The expression of Fas, FasL correlate with the clinicopathological parameters of laryngeal carcinoma significantly, which suggests that there is the loss of Fas and upregulation of FasL in the genesis and progression of laryngeal carcinoma. The detection of them is of important significance for evaluating the malignancy,prognosis of laryngeal carcinoma and directing the treatment.