三脉血注射液促进新生血管形成的实验研究

The experimental investigation on SanMaiXue injection enhancing neovascularization

目的 探讨三脉血注射液对新生血管形成的促进作用 ,从而为其应用于临床治疗冠心病提供实验依据。方法 利用大鼠主动脉段的离体培养 ,内皮细胞从动脉段切口处向外生长 ,形成具有分支结构的微血管样结构 ,于不同时间 ,在倒置显微镜下计数微血管样结构的数量 ,即能动态、定量的反映微血管生长的情况。结果 三脉血注射液及碱性成纤维细胞生长因子 (bFGF)均可产生特异性的血管生成作用 ,明显促进血管生长 ,明显增加新生毛细血管数目 (P <0 .0 1)。二者相比 ,前者促进血管生长作用强于后者 ,还可使血管新生作用提前 (P <0 0 1)。bFGF组对毛细血管的新生作用在一定范围内呈明显的剂量依赖关系 (P <0 .0 1) ,而三脉血组未发现此效应 (P >0 .0 5 )。结论 三脉血注射液对新生血管的形成有促进作用 ,其效应优于bFGF。

Objective It is to probe into the enhancing effect of Sanmaixue injection on neovascularization, and then provide the experimental basis for its treatment to coronary atherosclerotic heart disease. Methods Mice aorta was cultured in vitro. Endothelial cells grew out from the incision of aorta and formed micrangium structure with branching. The number of the micrangium structure was counted under the inverted microscope on different times. The growth state of micrangium reflected dynamically and quantitatively. Results Both Sanmaixue injection and bFGF have the specific effect on neovascularization. Both can enhance the blood vessel counts and promote the growth of new capillary significantly (P<0.01). Compared with bFGF, the effect of Sanmaixue injection on vasoformation was stronger and Sanmaixue injection could also make neovascularization genesis earlier (P<0.01). The effect of bFGF on vasoformation had obvious dependency relationship to its doses in limited range (P<0.01). The same phenomena was no found in Sanmaixue injection group (P>0.05). Conclusion Sanmaixue injection can promote the effect on neovascularization. It was better than bFGF.