摘要
为了进一步弄清高温抗条锈病的抗性机制,利用荧光显微技术和电镜技术研究了高温抗条锈性品种小偃6号的组织病理学特征和超微结构变化。结果表明:在高温抗条锈性表达时,病斑周围寄主细胞发生坏死和木质化,在高温处理24h,坏死细胞和木质化细胞数分别为初期的17.1和23.8倍。从超微结构观察,在高温抗条锈性表达时,侵染点附近寄主细胞壁表面凹凸不平、粗糙,细胞壁的厚度也较健康细胞厚。之后,细胞内的细胞器消解,仅有沿细胞壁分布的原生质和残存的一些颗粒状物质。
The characters of histopathology and the changes of ultrastructure of wheat leaves of Xi-aoyan No.6 were observed with fluorescence microscopy and electron microscopy to understand the mechanism of wheat high-temperature resistance to stripe rust. The result showed that the host cells necrosised and lignified in expression of wheat high-temperature resistance to stripe rust, and then the extension of hyphal in host cells was restrained. The necrosised and lignified cells increased 17.1 and 23.8 times respectively nindividually more than the original ones after 24 hours high temperature treatment. It was observed that the host cell wall became thicker than normal one, and the surface of the cell wall changed into roughness under electron microscopy. Afterwards the organelles in the cell vanished while protoplasm near the cell wall and some small substances as granule still existed.
出处
《植物保护学报》
CAS
CSCD
北大核心
2003年第2期119-124,共6页
Journal of Plant Protection
基金
国家自然科学基金(39170511)