灰树花胞外多糖的分离纯化及其性质研究

Studies on the Isolation,Purification and Characterization of an Extra cellular Polysaccharide from Grifola frondosa

灰树花发酵液浓缩 ,醇析后得多糖粗品。经H2 O2 脱色 ,Sevag法脱蛋白 ,透析 ,DEAESepharoseFastFlow柱层析纯化 ,得到灰树花胞外纯多糖GLP。HPLC检测为单一均多糖 ,其平均分子质量为 83 0 0 0。完全酸水解后经纸层析 ,气相色谱分析 ,GLP只含有葡萄糖。经红外光谱 ,刚果红实验 ,高碘酸氧化 ,Smith降解分析 ,初步推测其结构是具有 (1→ 6)和 (1→ 3 )

An extracellular polysaccharide GLP was isolated fro m the culture filtrate of Grifola frondosa.The crude preparation was obtained by alcohol precipitation and purifiedby ethanol grade fractionation and DEAE Sephar ose Fast Flow ion exchange chromatography. GLP was homogenous, as determined by HPLC and its averagemolecular weight was 83000. By PC and GC analyses, i t only contains glucose. IR, Cong Red reaction, periodate oxidation and Smith de gredation showed that GLP wasβ-glucan with (1→3) and (1→6)bonds.