脊髓型颈椎病椎间盘内白介素1β、肿瘤坏死因子α及一氧化氮的表达及其意义

Expression of IL-1β,TNF-α and nitric oxide in the cervical intervertebral disc of patients with cervical spondylotic myelopathy

目的 研究白介素 1β(IL 1β)、肿瘤坏死因子α(TNF α)及一氧化氮 (NO)在脊髓型颈椎病患者颈椎间盘组织内的表达 ,并探讨其意义。方法 收集 15例脊髓型颈椎病患者 (患病组 )行颈椎前路手术中切除的椎间盘组织 ,另取 6例无颈椎病史的颈椎外伤患者 (对照组 )术中切取的颈椎间盘作为对照 ,应用酶联免疫吸附测定法 (Elisa)测定椎间盘组织中IL 1β和TNF α的含量 ,以光密度法测定NO的含量。结果 脊髓型颈椎病患者颈椎间盘组织内IL 1β ,TNF α及NO的含量均明显高于对照组 (P <0 .0 1)。结论 颈椎间盘组织可自发产生IL 1β ,TNF α及NO ,而脊髓型颈椎病患者的颈椎间盘组织内上述物质的含量明显增高 。

Objective To study the expression of IL 1β,TNF α and nitric oxide (NO) in the cervical intervertebral disc and their roles in the pathogenesis of cervical spondylotic myelopathy (CSM). Methods Fifteen cervical discs were obtained from patients of CSM undergoing anterior disc surgery. Six cervical discs specimens were obtained in the surgical operation with patients with traumatic cervical injury served as the control. The specimens were analyzed for IL 1β、TNF α by use of ELISA method, NO was measured by optical density assays. Results The contents of IL 1β,TNF α and NO in disc of CSM patients increased significantly when compared with the control group. Conclusion The results of the present study indicated that IL 1β,TNF α and NO may be involved in the pathogenesis of CSM.