四氯化碳中毒对大鼠离体再生肝细胞钾离子外漏的影响

EFFECT OF CARBON TETRACHLORIDE INTOXICATION ON LEAKAGE OF INTRACELLULAR POTASSIUM IN REGENERATING RAT HEPATOCYTES IN VITRO

本工作用三种剂量四氯化碳(CCl_4,10,15和20mmol/L)损伤正常大鼠离体肝细胞,分别在5,10,15和20min测定细胞内K^+和GPT漏出量。实验观察到细胞内K^+和GPT漏出量与CCl_4染毒的剂量和时间有明显关系,而且K^+漏出量较GPT更能灵敏地反映细胞的损伤程度;用中等剂量CCl_4(15mmol/L)损伤离体再生肝细胞20min后,细胞内K^+漏出的变化百分数明显低于正常肝细胞。这些结果表明,大鼠离体再生肝细胞具有较强的抗CCl_4损伤作用,其机制可能与再生肝细胞膜稳定性较强有关。

Leakage of intracellular potassium and GPT in normal rat hepatocytes exposedto 10, 15 and 20 mmol / L of carbon tetrachloride (CCl_4) in vitro was determinedat 5, 10, 15 and 20 min after cell intoxication. Both of the intracellular potassiumand GPT were clearly dose- and time-dependent with CCl_4, but the leakage of in-tracellular potassium is a more sensitive index to indicate ccll injury than the later.The percent change of intracellular potassium in regenerating rat hepatocytes 20min after CCl_4 (15 mmol/L) intoxication was markedly reduced than that in nor-mal rat. This finding indicates that regenerating hepatocytes are resistant to CCl_4hepatotoxicity in vitro, and its mechanism presumably is due to a higher stabilityof regenerating liver cell membrane.