摘要
本文建立了大鼠垂体细胞灌流系统并利用这系统和放射免疫测定法检测了一些物质对垂体细胞促进或抑制ACTH的分泌作用。下丘脑抽提液能刺激垂体细胞分泌ACTH,并有明确的剂量反应关系;AVP,cAMP,Ca^(2+),K^+,去甲肾上腺素、灭吐灵和氟哌啶醇等对垂体细胞ACTH分泌也有兴奋作用。地塞米松和多巴胺能抑制垂体细胞ACTH的基础分泌并对抗兴奋性物质的作用;赛庚啶能选择性地拮抗某些兴奋性物质的作用,γ-氨基丁酸无明显抑制作用。
In addition to method of ACTH RIA, a rat pituitary cell perfusion systemwas developed for the assessment of pituitary cells in stimulating and inhibitingACTH secretion induced by some substances. Hypothalamic extract stimulatedthe ACTH secretion in a dose-dependent manner. AVP, cAMP Ca^(2+), K^+, nor-adrenaline, metoclopramide and haloperidol also had some stimulating effect. De-xamethasone and dopandne inhibited the basal ACTH secretion of pituitary celland antagonized the effect of the various stimulating substances. Cyproheptadinecould antagonize the effect of some of the stimulating substances while GABA hadno marked inhibiting effect.
出处
《生理学报》
CAS
CSCD
北大核心
1992年第4期414-419,共6页
Acta Physiologica Sinica
关键词
分泌调节
垂体细胞
ACTH
rat pituitary cell perfusion
adrenocorticotropin
regulation of secretion
