人子宫内膜纤蛋白溶酶元激活因子及其抑制因子的分布与调控

PLASMINOGEN ACTIVATORS AND PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 IN HUMAN ENDOMETRIUM

人子宫内膜中存在组织型(tPA)及尿激酶型(uPA)两类纤蛋白溶酶元激活因子,其含量在增殖期高于分泌期。本文应用免疫组织化学定位证实uPA及tPA两类抗原存在于子宫内膜的腺体细胞和间质细胞中。应用SDS-PAGE分高蛋白质,继而应用纤蛋白-琼脂糖铺盖技术测得离体培养下间质细胞仅释放tPA,腺体细胞仅释放uPA,但两种细胞均分泌PA的抑制因子(PAI)。培液中加入孕酮,明显抑制PA和刺激PAI生成。雌二醇作用与孕酮相反。某些肽类激素hCG、PRL、GnRH及cAMP作用基本与雌二醇相同。但福司克林(FK)则刺激间质、腺体两种细胞产生tPA及少量uPA,抑制PAI生成。本工作表明人子宫内膜中存在PA及PAI作用相反的酶,受激素调控,其生理意义尚待进一步探讨。

Two types of plasminogen activator (PAs) are present in human endometrium,and their contents vary with the different phases of menstrial cycle, i.e. high in theproliferative phase and low in the secretory phase. In the present study by im-munohistochemical technique, both uPA and tPA antigens were demonstrated inthe stromal and glandular cells of the endometrium. In cell culture, tPA wasreleased only from stromal cells and uPA only from glandular cells as determinedby SDS-PAGE followed by fibrin overlay technique, but PA inhibitor type-1 (PAI-1)was secreted by both stromal and glandular cells. Furthermore, secretion of PAsfrom endometrial cells was enhanced by adding estradiol and markedly inhibitedby progesterone in a dose dependent manner, while the PAI reacted just in theopposite way. The effect of the peptide hormones, hCG, GnRH, PRL, as wellas cAMP in cell culture on the sccretion of PAs and PAI was similar to that of es-tradiol, while forskolin demonstrated definitely more stimulative effect on tPAthan uPA. Taking into account of the finding of the present study, it appearsthat, under hormonal control, a balance between PAs and PAI in the endome-trium exists. The physiological roles of the PAs and PAI in the endometriumwere discussed.