摘要
目的 应用随机扩增多态DNA(RAPD)技术 ,对耐甲氧西林金黄色葡萄球菌 (MRSA)进行基因多态性研究。方法 对我院临床分离的 39株MRSA进行随机引物PCR扩增 ,扩增产物进行电泳和聚类分析。结果 39株MRSA经电泳得到 1～ 9条片段 ,经聚类分析可分为 8个型 ,位于我院东侧的 1、2和 6号楼的科室主要流行Ⅰ、Ⅱ、Ⅵ型 ,其中神经外科主要流行Ⅰ型 ,呼吸内科流行Ⅵ型 ;而位于我院西侧的 3、4、5号楼的科室主要流行Ⅲ、Ⅳ和Ⅷ型 ,其中综合 2科流行Ⅷ型。结论 通过RAPD分型研究 ,了解了我院MRSA基因型流行的特性 。
OBJECTIVE To investigate the genetic polymorphism of meticillin-resistant Staphylococcus aureus (MRSA) by randomly amplified polymorphic DNA (RAPD). METHODS The genes of MRSA were amplified by RAPD assay in 39 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis. RESULTS Nine amplified DNA fragments were observed in agarose gel and they were classified into 8 genotypes,which were in different geographical distribution. Genotypes Ⅰ,Ⅱand Ⅵ were mainly isolated from wards in east inpatient building, especially genotype Ⅰfrom the neurosurgery department and genotype Ⅵ from the respiratory department,while genotypes Ⅲ, Ⅳand Ⅷ were popular in wards in west inpatient building. Genotype Ⅷ was mainly in geriatric department. CONCLUSIONS RAPD assay reveals the special epidemic genotypes of MRSA in our hospital,which provides molecular epidemiological evidence for strategies to control MRSA infections.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第10期919-921,共3页
Chinese Journal of Nosocomiology
基金
辽宁省科技攻关基金资助项目 (0 0 2 2 5 0 0 2 )
