SARS病毒基因组所编码的E蛋白的二级结构和B细胞表位预测

Prediction of the secondary structure and B cell epitope for the E protein of SARS coronavirus

目的 预测SARS病毒E蛋白的B细胞表位和二级结构。方法 以SARS病毒基因组序列为基础 ,采用Gar nier Robson方法、Chou Fasman方法和Karplus Schultz方法预测E蛋白质的二级结构 ;用Kyte Doolittle方案预测蛋白质的亲水性 ;用Emini方案预测蛋白质的表面可能性 ;用Jameson Wolf方案预测氨基酸的抗原性指数。综合评判 ,预测SARS病毒E蛋白的B细胞表位。结果 在SARS病毒E蛋白N 端的第 1～ 6、13～ 19、39～ 4 3、4 7～ 6 4区段和第 73～ 76区段有 β 折叠中心 ;第 6～ 12区段和第 6 7～ 6 9区段可能形成转角或无规则卷曲 ,是柔性区域。E蛋白N端第 2～ 13区段和第 6 1～ 74区段为B细胞优势表位区域。结论 用多参数预测SARS病毒E蛋白的二级结构和B细胞表位 。

Objective To predict the secondary structure and B cell epitopes for E protein of SARS coronavirus. Methods The se- condary structure of E protein was predicted by the methods of Garnier-Robson,Chou-Fasman and Karplus-Schulz based on SARS coronavirus genome ,and hydrophilicity plot, surface probability plot and antigenic index were obtained by the methods of Kyte-Doolittle, Emini and Jameson-Wolf respectively. Combining the results according to these methods, the B cell epitopes for E protein of SARS coronavirus were predicted. Results There are some centers of β-sheet in the N-terminal No. 1-6,13-19,39-43,47-64 and 73-76. And the N-terminal No. 6-12 and 67-69 may be the flexible regions of E protein. The N-terminal No. 2-13 and 61-74 were the predominant epitopes of the B-cells.Conclusion Prediction of the secondary structure and B cell epitopes for the E protein by the multi-parameters is helpful for identification of B cell epitopes using experimental methods.