诸葛菜叶柄原生质体培养再生植株

Plant Regeneration from Petiole Protoplast Culture of Orychophragmus violaceus

本文首次报道用诸葛菜(Orychophragmus violaceus)试管苗叶柄为材料分离原生质体,经培养再生了植株。用于原生质体培养的基本培养基为Nitsch培养基,附加100mg/L丝氨酸,800mg/L谷氨酰胺和13%的蔗糖,激素成分为0.5mg/L BA,0.5mg/L NAA和1mg/L2,4-D(或0.5mg/L BA和2mg/L 2,4-D)。原生质体的培养密度为2×10~5/ml。培养7天的原生质体分裂频率约为40%。在附加0.05mg/L NAA和3mg/L BA的MS分化培养基上,愈伤组织可分化出大量的芽和苗,分化频率为100%。

Protoplasts were isolated from petioles of sterile seedlings 9f Orychophragmus violaceus. The medium for protoplast culture was Nitsch containing 100mg/L serine, 800mg/L glutamine, 13% sucrose and complemented with 0.5mg/L BA, 0.5mg/L NAA and 1mg/L 2,4-D(or with 0.5mg/L BA and 2mg/L 2,4-D). Protoplasts were cultured at a density of 2×10~5/ml. The frequency of protoplast division was about 40% after 7 days of culture. On MS medium containing 3mg/L BA and 0.05mg/L NAA, the protoplast-derived calli could regenerate many shoots and the regeneration frequency was up to 100%.