大白菜无菌苗叶肉原生质体植株再生

Plant Regeneration of Mesophyll Protoplasts From Axenic of Chinese Cabbage (Brassica pekinensis Rupr.)

商用大白菜叶肉原生质体,经液体培养基浅层培养,再生细胞分裂,并获得愈伤组织。愈伤组织转到分化培养基上诱导分化,已从城青2号大白菜叶肉原生质体得到再生的完整植株。再生细胞的分裂频率与培养基中的激素种类和浓度有关,受原生质体培养浓度的影响。多胺类物质[亚精胺(SPD)]的加入,可促进细胞分裂,并有益于以后植株的再生。通过提高新鲜培养液的pH值(6.5)可使细胞团的褐化得到明显的控制。在植株分化过程中,培养基中低浓度(1.1%)的蔗糖与植株分化有关。

Mesophyll protoplasts of commercial Chinese cabbage were cullured with liquid medium as shallow layer. Regenerated cells divided and calli were obtained. Regeneration plants of Chinese cabbage (Cheng Qing No.2) had been obtained from the regenerated calli after being transferred onto differentiation medium. The division frequency of the regenerated cells related to the kinds of the hormones and their concentrations, and was also influenced by the culture density of protoplasts. Addition of spermidine (SPD) into the protoplast culture medium enhanced cell division frequency and plant regeneration from the P-calli. By increasing the pH value (6.5) of fresh supplement protoplast culture medium, the 'cell browning' phenomenon was obviously controlled. In addition, the plant regeneration was favored by the presented low concentration of sucrose in the differentiation medium.