摘要
以人工合成的多肽PGAT为底物,鉴定了人外周血淋巴细胞膜TPK。首次发现IATPD对该酶活性有较强的抑制作用,其IC_(50)约为300μmol/L;改变其底物浓度,测定酶活性并应用Lineweaver-Burk双倒数作图法作图,结果表明,IATPD对该酶活性的抑制作用与ATP呈竞争性,与PGAT呈非竞争性。
In this paper, TPK activity from human peripheral blood lymphocyte membrane was measured using synthetic PGAT as substrate. The kinase activity was more strongly inhibited by a synthetic compound,1-isopropyl-6-amino-thieno (3,4-d) pyrimidine-2, 4-dione (IATPD); IC_(30) was about 300μmol/L. Lineweaver-Burk plots revealed that IATPD was competitively with ATP and noncompetitively with PGAT to inhibit the kinase activity.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1992年第3期190-194,共5页
Progress In Biochemistry and Biophysics
关键词
淋巴细胞
IATPD
酪氨酸
蛋白激酶
human peripheral blood lymphocyte membrane, TPK, IATPD, competitive inhibition, noncompetitive inhibition
