一种新型免疫亲和层析介质的研究

STUDY OF A NEW KIND OF IMMUNOAFFINITY CHROMATOGRAPHY MEDIA

以国产琼脂糖介质为原料,合成了含肼琼脂糖介质。抗体Fc段的糖基经氧化后,可与此种含肼琼脂糖介质偶联,制成高亲和率的免疫亲和层析介质。与溴化氰法相比,这种对抗体上的糖特异的偶联方法制成的免疫亲和层析介质,容量高、稳定性强。用这种含肼琼脂糖介质分别与肿瘤坏死因子和γ-干扰素等的抗体偶联,制成的免疫亲和层析介质可用于分离纯化相应的基因工程产物抗原。

Hydrazido-agarose was prepared and used for the site-specific immobilization of antibody. Antibody was oxidized with sodium periodate, resulting in the production of aldehyde on the carbohydrate moiety. The oxidized antibody was then reacted with the hydrazido-agarose to form stable hydrazone linkages The method of immobilization resulted in an increased activity and stability of the bound antibody. TNF, γ-IFN and IL-2 were purified by chromatography on immunosorbents prepared with monoclonal antibodies using this method respectively. Their activities were tested.