抗体包被瘤细胞膜引发人血多形核白细胞化学发光

CHEMILUMINESCENCE OF POLYMORPHONUCLEAR LEUKOCYTES INDUCED BY ANTIBODY-COATED TARGET CELL MEMBRANE

用抗体包被K562细胞膜碎片(Ab-M)刺激人血多形核白细胞(PMN)产生化学发光,对其发光动力学及活性氧代谢特征进行了比较,结果表明Ab-M引发PMN发光动力学与酵母多糖不同.用秋水仙碱干扰PMN膜结构完整性可抑制其发光产额,Ca^(2+)可促增PMN发光,提示PMN氧代谢的调控与Fc受体和Ca^(2+)动员有关;活性氧系PMN实施细胞毒效应的重要物质.

Polymorphonuclear leukocytes (PMN)and soluble or particulate matter interact, to produce chemiluminescence (CL)which links to activation of the oxidative metabolism of the cells. While studying the characteristics of the CL induced by antibody-coated crude membrane fragments of K562 tumor cells, the result was observed that PMN were not triggered to a CL response in the absence of antibodies or the fragments. Furthermore, the fact that Cl could be inhibited by colchicine which disturb the integrity of the PMN cytoskeleton, indicate that the cytoskeleton is linked to Fc receptor binding or signal tra- nsduction. The luminol-enhanced CL was dependent on the presence of calcium and hydroxyl radicals were involved.