中国人肿瘤细胞中O^6-甲基鸟嘌呤DNA甲基转移酶活性与对嘧啶亚硝脲敏感性的关系

O^6-Methylguanine-DNA methyltransferase Activity and Sensitivity of 20 Chinese Tumor Cell Strains to ACNU

分析了20株中国人肿瘤细胞的O^6-甲基鸟嘌呤DNA甲基转移酶(O^6-MT)活性及对嘧啶亚硝脲ACNU的敏感性,发现两者间有较好的线性关系,O^6-MT低,对ACNU敏感,提示O^6-MT可作为使用ACNU对肿瘤化疗时的一项予见性指标。

O6-methylguanine-DNA methyltransferase (O6-MT) plays an important role in repairing alkylated DNA.O6-MT activity as well as cellular sensitivity to ACNU of 20 Chinese tumor cell strains were assayed.A linear response between O6-MT activity and ACNU sensitivity (D10) was observed.The lower the O6-MT activity in the cells, the more the sensitivity to ACNU killing.It suggested that assay of O6-MT activity in tumor biopsy could be used as a guide to predict the effectiveness of ACNU treatment in chemotherapy of human cancer.