用甲酰胺提高多聚酶链式反应的特异性、灵敏度和效率的研究

Increase of Specificity, Sensitivity and Efficiency of PCR Reaction by the Addition of Formamide

在多聚酶链式反应体系中加入甲酰胺可以有效地抑制非特异性扩增,提高特异性扩增,并使反应的灵敏度提高一个数量级。

Addition of formamide to PCR system will efficiently inhibit non-specific amplification, increase specific amplification and sensitivity up to an order of magnitude.