人膀胱癌中某些癌基因的研究

Expression and Regulation of some Oncogenes in Human Bladder Cancer

本实验应用Northern和斑点印渍杂交技术探测了人膀胱癌细胞株中c-myc、c-fos、erbB等癌基因的表达,以及TPA对这些癌基因表达的调控,发现BIU-87细胞有这些癌基因的表达,并能被TPA所增强,同时也发现人膀胱癌组织有c-myc、c-fos、erbB、N-ras基因的高表达。提示蛋白激酶C的激活可以诱导某些癌基因的表达。多种癌基因的表达异常可能在膀胱癌中起重要作用。

Using Northern and dot blot analysis, we investigated the expression of c-myc, c-fos abd erbB and regulation of oncogenes by TPA in BIU-87 cells a human bladder transitional cell carcinoma line We found expression of these oncogenes and induc-tion of these genes by TPA.We also found high expression of c-myc, c-fos, erbB, N-ras in human bladder cancer tissues.In contrast, only low level expression of N-ras was detected in normal bladder tissues.These data suggest that the expression of some oncogenes can be modulated in a protein kinase C dependent manner and high level expression of some oncogenes are responsible for the abnormal growth in bladder carcinoma.