胃粘膜良恶性病变中P53和bc1-2表达的研究

A study on the expression of P53 and bc1-2 in both benign and malignant lesion of gastric mucosa.

目的 探讨P5 3与bc1- 2基因蛋白在胃癌及癌前病变组织中的表达特点及相关性。 方法 收集活检胃粘膜标本及部分手术切除胃癌病理标本 12 6例 ,其中浅表性胃炎 (CSG) 2 9例 ,萎缩性胃炎 (CAG) ,肠上皮化生 (IM) ,不典型增生 (DyS)各 2 0例 ,胃癌 (GC) 37例。应用SP免疫组织化学方法检测P5 3、bcl- 2基因蛋白的表达。 结果 P5 3在CSG、CAG、IM、DyS、GC中的阳性表达率分别为 6 9% (2 /2 9)、10 % (2 /2 0 )、15 % (3/2 0 )、30 % (6 /2 0 )、45 95 % (17/37)。bc1- 2在上述各组中的阳性表达率分别为10 34% (3/2 9)、10 % (2 /2 0 )、2 0 % (4/2 0 )、2 5 % (5 /2 0 )、40 5 4% (15 /37)。P5 3、bc1- 2表达阳性率GC组显著高于CSG组 (P <0 0 1) ,高于IM组 (P <0 0 5 ) ,IM、DyS、GC组P5 3、bc1- 2有共同表达 ,差异无显著性 (P>0 0 5 ) 结论 P5 3和bc1-

Ojective To study on the expression and Corelation of P53 and bc1-2 protein in gastric cancer and gastric precancerous lesions. Methods P53 and bc1-2 protein were determined with immunohistochemical techinque in 29 cases of superficial gastritis (CSG), 20 cases of atrophic gastritis (CAG), 20 cases of intestinal metaplasia (IM), 20 cases of dysplasia (DyS) and 37 cases of gastric cancer (GC) tissue specimens. Results The positive rate of P53in CSG、CAG、IM、DyS and GC was 6.9%(2/29)、10%(2/20)、15%(3/20)、30%(6/20)、45 95%(17/37),respectively. The positive rate of bc1-2 in GSG、CAG、IM、DyS and GC was 10.34%(3/29)、10%(2/20)、20%(45/20)、25%(5/20) and 40.54%(15/37), respectively. The positive rate of both P53 and bc1-2 in GC was significantly higher than that in CSG (P<0.01) and higher than that in IM (P<0.05). There was positive expression on both P53 and bc1-2 in IM, DyS and GC(P>0.05) Conclusions The total positive rate of P53 and bc1-2 in GC is significantly higher than that in benign lesion.