人参细胞悬浮培养中的激素调节及细胞分裂的研究

Studies on Hormone Regulation and Cell Division in Cell Suspension Culture of Panax Ginseng GAMey

在人参细胞悬浮培养中,单使用植物生长调节剂时以Zmg/L 2,4—D和2mg/LNAA的效果较好.在不同激素组合的试验中,以2mg/L 2,4—D+2mg/L NAA+0.5mg/L KT的效果最好.悬浮条件下人参细胞的再生方式有有丝分裂、无丝分裂和出芽繁殖,其中直径为50μm以下的小型园形细胞的分裂为有丝分裂,而葫芦形、肾形、长园形及不定形的巨型细胞常表现为无丝分裂和出芽繁殖.植物激素对不同类型细胞的分裂有一定的影响作用.和愈伤组织固体培养相比,细胞悬浮培养可使细胞干重增加近5倍.

The experimental results of ginseng cell suspension culture showed that, when used singly plant hormone 2mg/L2, 4-D and 2mg/L NAA were satisfa-ctory when used combination of hormone, 2mg/L 2,4-D + 2mg/L NAA + 0.5 mg/L KT were the most effective in. promting the growth of suspension cell Reproduction of ginseng cells include amitosis ritosis germinal reproduc-tion Small-type cells below 50um diater are mitosis and big-type cells are amitosis and germinal reproduction Hormones affect different type cells divis-ion Cell dry weight of ginseng suspension cell culture increases about five times than callus solid culture.