期刊文献+

Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage A in a Novel Recombinant Escherichia Coli Used for Production of PHB 被引量:2

Simultaneous Expression of Vitreoscilla Globin Gene and Lytic Genes of Phage λ in a Novel Recombinant Escherichia Coli Used for Production of PHB
下载PDF
导出
摘要 Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage A with S amber mutation (S-RRz) and poly(B-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coli cell, simultaneously or respectively. Six novel strains containing phbCAB and vgb with or without lytic genes were constructed. Strain VG1 (pTU14), in which vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB without S- RRz were not better than that of strains harbored ph&CAB only. The simultaneous expression of vgb and S- RRz in the recombinant VG1 (pTU14) showed a great potential for low-cost production of PHB. Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage γ with S amber mutation (S^-RRz) and poly(fl-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coil cell,simultaneously or respectively. Six novel strains containing phbCAB and vgb with or without lytic genes were constructed. Strain VG1 (pTU14), in which vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB without S- RRz were not better than that of strains harbored phbCAB only. The simultaneous expression of vgb and S-RRz in the recombinant VG1 (pTU14) showed a great potential for low-cost production of PHB.
出处 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2001年第4期407-411,共5页 中国化学工程学报(英文版)
基金 Supported by the National Natural Science Foundation of China (No. 29834103, 29876021).
关键词 Vitreoscilla hemoglobin lytic genes of phage λ poly (β-hydroxybutyrate) 透明颤菌血红蛋白基因 γ噬菌体裂解基因 化工生产 PHB 细胞重组 大肠杆菌 同时表达 聚(β-羟基丁酸酯) 生物合成
  • 相关文献

参考文献1

  • 1Yu H M,J Biosci Bioeng,2000年,89卷,4期,307页

引证文献2

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部