4-异硫氰酸盐-2,2,6,6-四甲基哌啶-1-氧自由基抑制白血病细胞DNA,RNA,蛋白质,核蛋白和ATP代谢的动力学研究

A KINETIC STUDY OF INHIBITION EFFECT OF 2,2,6,6TETRAMETHYL-4 ISOTHIOCYANO-PIPERIDINE-1-OXYL ON SYNTHESIS OF DNA, RNA, PROTEIN, NUCLEOPROTEIN AND ATP IN LEUKEMIA 7712 CELLS

4—异硫氰酸盐—Tempo 3μg/ml对白血病7712细胞DNA,RNA和蛋白质合成抑制的动力学研究表明,首先抑制DNA的合成,然后逐步抑制RNA与蛋白质的合成。对18种氨基酸代谢均有抑制作用,大部分抑制率在30％左右,唯对蛋氨酸的抑制率高达45.8％,似有一定选择性。对核蛋白与ATP代谢的抑制动力学过程,有较好的相关性,表明其对癌细胞生长及DNA合成的抑制作用与对细胞ATP合成的抑制有关。

A kinetic study on 4-isothiocyano-piperidine-tempo 3 μg/ml inhibiting synthesis of DNA, RNA,and protein of leukemis 7712 cells showed that it first inhibited synthesis of DNA, and then gradually synthesis of RNA and protein (Fig.1) It had inhibition effects on 18 kinds of amino acid of cancer cells. The inhibition % on methionine was 45.8%, the rest about 30% which seem ed to have a preferential choice. It had some relationship with the A correlation of inhibition on nucleoprotein with ATP, suggested that the inhibition effect on DNA synthesis and growth of cancer cells was related to that of ATP synthesis (Fig.2).