摘要
应用ESR和自旋捕集相结合的技术直接测定了过硫酸铵—N,N,N′,N′-四甲基乙二胺(AP-TEMED)体系产生的氧自由基,经计算机波谱模拟和计算波谱参数证实该体系产生的氧自由基是O_2^-和·OH。并用维生素C、茶多酚、超氧化物歧化酶等氧自由基清除剂,从聚丙烯酰胺凝胶法、化学发光法和脂质过氧化法不同角度研究了AP-TEMED体系在自由基研究方面的应用意义。
Oxygen radicals generating in AP-TEMED system measured directly by ESR and spin trapping methods were distinguished as 0_2^(-) and ·OH by spectra of computer simulation and parameters calculated from the spectra. The system of AP-TEMED used in the study on scavenging of radicals by vitamin C, green tea polyphenols and superoxide dismutase was appraised by approaches, such as polyacrylamide gel, luminol mediated chemiluminescence and inhibition of lipid peroxidation.
出处
《生物物理学报》
CAS
CSCD
北大核心
1992年第2期334-338,共5页
Acta Biophysica Sinica
关键词
自旋捕集术
氧自由基
AP-TEMED
spin trapping medhods, Oxyen radicals, AP-TEMED (ammoniun persulfate-N,N',N,N'- tetramethvl ethylendiamine) system.