摘要
付里叶变换红外光谱法测定了胰岛素的二级结构,讨论了信/噪比、谱解析、曲线拟合和吸收组分的指认对结构测定的影响。测定的胰岛素二级结构与X—光结构分析结果吻合。
The secondary structure of insulin on the dried film was determined by means of Fourier transform infrared spectroscopy. The problems related to the determination, such as the signal/noise ratio, resolution enhancement of the spectrum, curve fitting and the assignment of the absorption components were discussed. The contents of the substructures thus determined are in agreement with those derived from X-ray data.
出处
《生物物理学报》
CAS
CSCD
北大核心
1992年第2期312-317,共6页
Acta Biophysica Sinica
基金
国家自然科学基金
中关村分析测试基金资助课题
关键词
胰岛素
红外光谱
蛋白
二级结构
Insulin, FTIR, Secondary structure of protein.
