茶多酚单体L-EGCG对气相烟引起鼠肺细胞膜损伤的抑制作用

INHIBITION EFFECT OF L-EGCG ON CIGARETTE SMOKE-INDUCED DAMAGE TO RAT LUNG CELLS

茶多酚(GTP)单体L-EGCG对香烟气相物质损伤鼠肺细胞膜的保护作用研究结果表明,L-EGCG能抑制香烟气相物质引发的脂质过氧化;用脂肪酸自旋标记物5-DOXYL和16-DOXYL标记鼠肺细胞膜,发现预先加入L-EGCG可抑制气相烟引起的膜浅层流动性改变,并与L-EGCG的浓度呈量效关系。在0.001到0.1mg/mL浓度范围内,L-EGCG本身对膜的浅层没有影响,而能使膜深层的流动性略有增大。由试验推测,L-EGCG的保护作用很可能是由于清除了气相烟中的自由基或脂质过氧化产生的脂类自由基。

The role of L-EGCG [(-)——epigallocatechin-3-gallate, one of major co- mponents of green tea polyphenols(GTP)] in the damage process of gas-phase cigarette smoke (GPCS)to rat lung cells (RLC) was studied. The results showed that L-EGCG was able to inhibit GPCS-induced damages, e.g., lipid peroxidation and alteration of membrane fluidity of RLCs. When the concentration was between 0.001 and 0.1 mg/ml, L-EGCG had little effect on the shallow-layer of membrane, but slightly increased membrane fluidity of the deep-layer. It was presumed that the protection effect of L-EGCG in the system might be carried out by scavenging free radicals in GPCS and lipid free radicals from lipid peroxidation.