巨噬细胞对氧化和丙二醛修饰的低密度脂蛋白结合与降解特性的比较研究

COMPARATIVE STUDY OF BINDING AND DEGRADATION OF OXIDATIVELY AND MALONDIALDEHYDE MODIFIED LOW DENSITY LIPOPROTEIN BY MACROPHAGE

用Cu^(2+)(引发氧化修饰)和脂质过氧化降解产物丙二醛对低密度脂蛋白(LDL)进行修饰,分别测定了巨噬细胞系P^(300)D_1和小鼠腹腔巨噬细胞对两种被修饰LDL的结合量(包括内移量)和降解量。结果显示:LDL经氧化修饰和丙二醛修饰后被两类巨噬细胞的结合量与降解量均高于正常LDL,在修饰程度相近(琼脂糖电泳迁移率相近)时,两类巨噬细胞对氧化修饰LDL的结合量与降解量高于丙二醛修饰的LDL。竞争性抑制结果显示,丙二醛修饰的LDL和乙酰化修饰的LDL均可部分抑制巨噬细胞对氧化修饰LDL的结合与降解。

In this paper, LDL was modified by Cu2 + (initiating lipid peroxidation) and malondialdehyde. The binding and degradation of two kinds of modified LDL by macrophage cell line P388D1 and mouse peritoneal macrophage were determined. The results showed that the binding and degradation of Cu2+ and MDA modified LDL by two kinds of macrophages were higher than that of native LDL. When the modification degree was similar, the binding and degradation of oxidatively modified LDL by two kind of macrophage was more than that of MDA modified LDL. Competitive inhibition showed that the binding and degradation of oxida-tively modified LDL by macrophage may be partly inhibited by MDA modified LDL or acetylated LDL.