肾性贫血红细胞膜蛋白巯基结合位置性质的ESR研究

ESR STUDLES ON THE PROPERTLES OF SULFHYDRYL BINDING SITES OF THE RED BLOOD CELL MEMBRANES OF PATLENTS WTTH ANEMIA OF CRF

用四种不同链长的马来酰亚胺氮氧自由基标记物研究了慢性肾衰竭(CRF)贫血病人红细胞膜蛋白巯基结合位置的性质。从所得的ESR波谱计算了弱、强固定化成分之比(W/S)。结果表明,由M(Ⅰ)、M(Ⅱ)和M(Ⅲ)标记病人红细胞膜所得的W/S值都比标记正常人红细胞膜得到的W/S位高(P<0.05),这说明慢性肾衰竭贫血病人红细胞膜蛋白的巯基结合位置的构象发生了变化;用M(Ⅳ)标记正常人红细胞和病人红细胞所得到的ESR波谱都只含有弱固定化波谱,没有强固定化波谱。得出的这个结果将为探讨CRF贫血的发病机理提供理论证据。

The properties of sulfhydryl binding sites of membrane proteins in the erythrocytes of patients with anemia of chronic renal failure(CRF) were studied with four maleimide Spin labels with different chain lengths. The ratios of weakly immobilized component to the strongly immobilized component(W/S) were calculated on the basis of ESR spectra. The values of W/S from patient erythrocytes labeled with M(Ⅰ), M(Ⅱ), and M(Ⅲ) were higher than those of normal controls. The results indicated that the structure of the sulfhydryl groups in the erythrocytes of patients have been changed as a result of anemia of CRF. These findings provide a theoretical evidence for studying the pathoge-nesis and clinical treatment of the anemia of chronic renal failure.