微波辐射对小鼠脑内乙酰胆碱含量及胆碱乙酸转移酶活性的影响

EFFECTS OF MICROWAVE IRRADIATION ON ACETYLCHOLINE CONTENT AND CHOLINE ACETYLTRANSFERASE ACTIVITY IN MOUSE BRAIN

用频率为2450MHz功率密度为10mW/Cm^2(WBASAR约11.4W/kg)的微波(连续波)对置于微波暗室内的昆明种雄性小鼠急性全身照射1小时后,立即按常规方法断头,取脑,制成样品,然后用放射免疫测定法测量小鼠脑内乙酰胆碱(ACh)含量及胆碱乙酰转移酶(ChAT)活性。结果表明:照射组的ACh含量为11.6±1.4pmol/mg(脑鲜重),ChAT活性为45.4±8.7pmolACh/min.mg(脑鲜重);而对照组的分别为16.0±2.1pmol/mg和61.0±13.8pmolACh/min.mg。证明微波照射后可引起动物脑内ACh水平和ChAT活性下降,提示微波辐射对中枢胆碱能系统确有不利影响。

Male Kunming mice (18-20 g) were ac utely irradiated in a screened chamber with continuous waves of 2450 MHz microwave at power density of 10mW/ cm2 (WBASAR of about 11.4W/Kg) for 1 hr. Immediately after irradiation, the decapitation of the animals, the removal of brains and the preperation of samples were rapidly carried out with [he conventional method. Then brain ace tvlcholine (ACh) contents and choline acetyltransferase (ChAT) activities were determined by the radioimmunoassay. The ACh content and the ChAT activity in the mouse brain were found to be 11.6 1.1 pmol/mg (wet brain weight) and 45.4±8.7 pmol ACh/min ·mg (wet brain weight) after irradiation, respectively. In control mice, the ccmparable values were 16.0±2.1 and 61.0±13.8, respectively. It showed that exposure to microwave irradiation might cause the reduction of the ACh level and the ChAT activity in the animal brains, which suggested that adverse influences of the microwave radiation on the central cholinergic system did exist.