摘要
将经过系统鉴定的 2株鸡肾型传染性支气管炎病毒 (NIBV)在鸡胚肾细胞上盲传 7代后 ,均可不同程度的产生特征性细胞病变效应 (CPE) ,表现为细胞膜界限消失及多核巨细胞的形成 ,并通过间接免疫荧光抗体染色法 ,免疫复合物电镜观察及姬姆萨染色法对 CEK适应毒进行鉴定 ,结果表明 2株 NIBV鸡胚适应毒经多次传代后均适应于
Two strains of identified as avian nephropathogenic infectious bronchitis virus (NIBV) produced cytopathic effect(CPE)after 7 passages in chicken embryo kidney cell ,and showed the syncytial type of CPE in which the infected cells coalesced with loss of cell boundaries and revealed multinucleated giant cells. The replication of two strains of NIBV in Chicken Embryo Kidney Cells(CEK) were verified by indirect immunofluorescent antibody metheod ,by immunoelectronic microscope technology,by Gimsa stain method. The result suggest that two strains of Chicken Embryo (CE) adapted NIBV were adapted to replicate in CEK by many passages in CEK.
出处
《河南职业技术师范学院学报》
2002年第4期49-52,共4页
Journal of Henan Vocation-Technical Teachers College
基金
北京市科委资助项目 (85 41 3 0 3 0 0 )
