生长抑素与大肠癌细胞凋亡及调控基因bcl-2、bax及p53关系的研究

Investigation of the correlation between expression of somatostatin and cell apoptosis,regulation of gene bcl-2,bax and p53 in large intestine carcinoma

目的 探讨生长抑素 (SS)与大肠癌细胞凋亡指数 (AI)和凋亡调控基因p5 3、bcl 2、bax的关系。方法 采用免疫组织化学SABC法和分子生物学细胞原位凋亡检测技术中的TUNEL法 ,检测 6 2例大肠癌组织中SS、p5 3、bcl 2、bax及细胞凋亡的表达情况。结果 在大肠癌组织SS高表达组、中表达组的AI明显高于SS低表达组 (P <0 .0 1) ;p5 3、bcl 2、bax阳性表达率在SS低表达组、中表达组、高表达组三组间相比较存在着明显差别 (P <0 .0 5 ) ,其中bax在SS高表达组、中表达组的阳性表达率明显高于低表达组 (P <0 .0 5 ) ;bcl 2与其相反。p5 3在SS高表达组的阳性表达率明显低于低表达组 (P <0 .0 5 ) ;而p5 3在SS中表达组表达的阳性表达率低于低表达组 ,但其统计无明显差别 (P >0 .0 5 )。结论 生长抑素对大肠癌细胞凋亡的调控可能与 p5 3、bcl 2。

Objective To explore the correlation between somatostatin(SS) and apoptosis index(AI),the large intestine cancer cell apoptosis and the regulation of gene p53,bcl-2,bax. Methods The expression of SS, p53,bcl-2,bax and apoptosis cells were detected by immunohistochemistry(Streptavidin-Biotin-peroxidase Complex, SABC) and molecular biology in situ apoptosis detecting technic (TUNEL) methods. Results The AI in SS high and middle expression set of large intestine cancer was remarkably higher than that in low expression set(P<0.01). The positive expression rate of bax, bcl-2, p53 had prominent difference in SS high and middle and low expression groups(P<0.05).The positive expression rate of bax was higher in SS high and middle expression set than that in low expression set(P<0.05); but the expression of bcl-2 was opposite. The positive expression rate of p53 was lower in SS high expression set than that in low expression set(P<0.05);That of middle expression set was lower than that in low expression set,but it was not significant in statistic(P>0.05).Conclusion The regulation and control of the SS to the cell apoptosis of large intestine carcinoma may be related to the anomalous expression of gene,bcl-2,bax and p53.