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人端粒重复序列结合因子基因组和假基因研究 被引量:1

Study on the genome structure of human telomeric repeat binding factor 1 and its pseudogenes
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摘要 目的 :确定人端粒重复序列结合因子 (TERF1)的基因组结构和假基因的定位与结构。方法 :利用 Gen Bank等生物信息学资源 ,NCBI提供的 BLAST及其他相关的生物信息学工具 (Sequencher,DNA Strider和 Autoassembler等 ) ,确定 TERF1基因组和假基因的定位与结构 ,并用 PCR和测序证实。结果 :定位在 8q13上的 TERF1基因组由 10个外显子构成。它有 4个假基因分别分布在第 13、18、2 1和 X染色体上 (Ψ TERF1- 13、Ψ TERF1- 18、Ψ TERF 1- 2 1和Ψ TERF1- X) ,其结构均与 TERF1m RNA相似 ,但缺少部分外显子。Ψ TERF1- 13、Ψ TERF 1- 18和Ψ TERF1- 2 1的周边序列之间存在长度至少 6 0 kb的同源片段。结论 :TERF1基因组含 10个外显子 ,它有 4个加工过的假基因分布在第13、18、2 1和 X染色体上。大片段同源序列属近期重复片段中的染色体间倍增。 Objective: To determine the genome structure of human telomeric repeat binding factor 1 (TERF1) and its pseudogenes. Methods: Sequences were obtained from GenBank and analyzed using the BLAST program and other relevant biology program (Sequencher,DNA Strider and Autoassembler,etc) to determine the genome and pseudogenome structure of TERF1. PCR and sequencing were performed to verify the results. Results: TERF1 gene,which mapped to 8q13 was divided into 10 exons. It had four processed pseudogenes located on chromosome 13, 18, 21 and X respectively(ΨTERF1-13,ΨTERF1-18,ΨTERF1-21 and ΨTERF1-X). They were entire intronless TERF1 genes which lacked some exons. Three homologous fragments of at least 60 kb on the flanking region of ΨTERF1-13, ΨTERF1-18 and ΨTERF1-21, respectively were noted. Conclusion: TERF1 gene has 10 exons. It has four processed pseudogenes which are located on chromosome 13, 18, 21, and X, respectively. Large homologous fragments that belong to the recently duplicated segments are transchromosomal duplications.
出处 《浙江大学学报(医学版)》 CAS CSCD 2003年第5期407-411,共5页 Journal of Zhejiang University(Medical Sciences)
基金 国家自然科学基金 (39870 339) 浙江省自然科学基金 (39914 1)
关键词 端粒重复序列结合因子 外显子 假基因 Telomeric repeat binding factor Exon Pseudogene
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参考文献14

  • 1BLACKBURN E H. Structure and function of telomeres[J]. Nature, 1991,350(6319) :569-- 573.
  • 2SHIPPEN-LENTZ D, BLACKBURN E H. Functional evidence for an RNA template in telomerase[J]. Science, 1990,247(4942):546--552.
  • 3KIM N W, PIATYSZEK M A, PROWSE K R, et al.Specific association of human telomerase activity with immortal cells and cancer[J]. Science, 1994, 266(5193) :2011--2015.
  • 4CHONG L, STEENSEL B V, BROCCOLI D, et al. A human telomerie protein[J]. Science, 1995, 270(5242) : 1663-- 1667.
  • 5YAMADA K,YAJIMA T,YAGIHASHI A,et al. Role of human telomerase reverse transcriptase and telomerit-repeat binding factor proteins 1 and 2 in humanhematopoietic cells[J]. Jpn J Cancer Res, 2000, 91(12) : 1278-- 1284.
  • 6ARAGONA M, BUDA C A, PANETTA S, et al. Immunohistoehemical telomerie-repeat binding factor-1 expression in gastrointestinal tumors [J]. Oncol Rep,2000,7(5) :987--990.
  • 7KISHI S, WULF G, NAKAMURA M, et al. Telomeric protein Pin2/TRF1 induces mitotic entry and apoptosis in cells with short telomeres and is down-regulated in human breast tumors[J]. Oneogene, 2001,20 (12) :1497-1508.
  • 8ARAGONA M, PONTORIERO A, PANETTA S, et al. The role of telomere-binding proeins in carcinogenesis[J]. Minerva Med, 2000,91(11--12) :299--304. [inItalian].
  • 9BROCCOLI D, CHONG L, OELMANN S, et al. Comparison of the human and mouse genes encoding the telomeric protein, TRFI: chromosomal localization, expression and conserved protein domains[J]. Hum Mol Genet, 1997,6(1):69--76.
  • 10YOUNG A C, CHAVEZ M, GIAMBERNARDI T A,et al. Organization and expression of human telomere repeat binding factor genes[J]. Somat Cell Mol Genet, 1997,23(4) :275--286.

同被引文献8

  • 1蓝建平,来晓瑜,朱园园,孙洁,李静远,余建,谭亚敏,施继敏,林茂芳,黄河.人端粒结合因子在端粒酶阳性和阴性细胞中的定位与周期性表达的研究[J].浙江大学学报(医学版),2004,33(6):475-480. 被引量:2
  • 2孙洁,来晓瑜,朱园园,蓝建平,汤立旦,李静远,余建,谭亚敏,林茂芳,黄河.人端粒重复序列结合因子在急性白血病细胞中的表达及与端粒酶活性的关系[J].浙江大学学报(医学版),2004,33(6):491-495. 被引量:2
  • 3施继敏,丁伟,黄河,张志根,汤立旦,林茂芳.端粒重复序列结合因子在肾脏肿瘤中的表达水平[J].浙江大学学报(医学版),2004,33(6):496-499. 被引量:3
  • 4GU J,KAGAWA S,TAKAKURA M,et al.Tumor-specific transgene expression from the human telomerase reverse transcriptase promoter enables targeting of the therapeutic effects of the Bax gene to cancers[J].Cancer Res,2000,60:5 359-5 364.
  • 5TILLMAN D M,IZERADJENE K,SZUCS K S,et al.Rottlerin sensitizes colon carcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis via uncoupling of the mitochondria independent of protein kinase C[J].Cancer Res,2003,63(16):5 118-5 125.
  • 6ASHKENAZI A,PAI R C,FONG S,et al.Safety and antitumor activity of recombinant soluble Apo2 ligand[J].J Clin Invest,1999,104:155-162.
  • 7JO M,KIM T H,SEOL D W,et al.Apoptosis induced in normal human hepatocytes by tumor necrosis factor-related apoptosis-inducing ligand[J].Nature Med,2000,6:564-567.
  • 8NAKAMURA T M,MORIN G B,CHAPMAN K B,et al.Telomerase catalytic subunit homologs from fission yeast and human[J].Science,1997,277:955-959.

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